Brucellosis is a neglected tropical food-borne zoonotic disease with a high prevalence in many developing countries and has a negative impact on both animal and human health. Pig brucellosis is emergent and neglected in Cameroon. However, there is a paucity of data at the national level and risk factors associated with the disease are not well known. A slaughterhouse study was conducted at three Municipal abattoirs in Yaounde to determine the seroprevalence, assess the risk practices and evaluate the usage of personal protective equipment (PPE) by abattoir workers againts Brucella infection. A cross-sectional study was conducted from May 2020 to January 2021. Serum samples from 384 slaughtered pigs at three municipal abattoirs in Yaounde were collected and screened for anti-brucella antibodies using Rose Bengal Plate Test (RBPT) and indirect ELISA test. Furthermore, 41 plausible exposed and vulnerable workers to brucellosis (4 veterinarians; 23 slaughteres and 15 butchers) were recruted to evaluate the perception about the usage of PPE, length of employment and their level of knowledge about zoonotic diseases. All collected data were statistical analysed using GraphPad Prism 8.1. Among the 384 serum sampled, 11(2.86%) and 23 (5.98%) were positive for Brucella infection to Rose Bengal Plate Test (RBPT) and indirect Enzyme Linked Immuno-Sorbent Assay (iELISA) respectively. Moreover, 10 (2.6%) sera were positive to both tests, 13 (3.38%) positive to ELISA but negative to RBPT. Only one serum (0.26%) was positive to RBPT but negative to ELISA. None of the abattoir workers used standard protective coveralls. The boots were principaly used by the slaughterers. In general, three quarter of respondents did not know brucellosis and they were not educated. This study showed that the prevalence of Brucella infection was low among slaughtered pigs in Yaounde abattoirs.