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Initial Phytochemical Screening of Moringa Oleifera Extract Using Different Solvent


Author: Sutrisno Adi Prayitno*, Sugiyati Ningrum, Silvy Novita Antrisna Putri, Dwi Retnaningtyas Utami
Published Date: 2024-03-12
Keywords: Extraction; IC50; maceration; moringa; phytochemicals; solvent.
Abstract:
Moringa oleifera is a genus of plants that play an important role in human health. Moringa oleifera includes a variety of nutrients, including vitamins, amino acids, minerals, phenolic compounds, flavonoids, and antioxidants. To obtain these, do an extraction technique with the necessary demands and conditions. The study aim was to determine the total phenols, total flavonoids, and antioxidants (IC50) of Moringa leaf powder extract using first phytochemical screening. The extraction method is maceration with various solvents, and the phytochemical assays are carried out using the Folin Ciocalteu, quercetin standard, and DPPH methods. The results showed that the different solvents used had an effect on phytochemicals (TPC, TFC, and antioxidants). Macerated extraction of Moringa leaf powder with ethanol solvent produced 22.71 mg QE/g, 63.85 mg GAE/g, and an IC50 of 118.19 ppm (μg/L). The hexane solvent produced 31.23 mg QE/g of total flavonoids and 331.4 mg QE/g of total phenol, with an IC50 of 351.39 ppm (μg/l). The phytochemical extraction process is regulated by the extraction technique and solvent. The chemicals dissolved in the solvent reveal the polarity and type of substance suitable for the solvent. The extraction method works on the premise of "like dissolves like," producing extracts and bioactive components (phytochemicals) that are appropriate for the purpose.

Journal: ISAR Journal of Agriculture and Biology
ISSN(Online): 2584-217X
Publisher: ISAR Publisher
Frequency: Monthly
Language: English

Initial Phytochemical Screening of Moringa Oleifera Extract Using Different Solvent
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